9/6/2023 0 Comments Snoop catcher uwThe same Oxford research group have solved the problem by developing SnoopLigase. Moreover, when the Tag/Catcher systems are used for vaccine optimisation, the induction of antibodies or T cells may be directed towards the Catcher rather than the target antigen. For example, the Catchers need to be fused at the termini of proteins to prevent protein folding interferences. The functionality of the tools is, however, limited by the size of the Catcher – the partner protein for the corresponding Tag peptide. The Tag/Catcher technologies are currently used by researchers worldwide. The Tags and Catchers act as ‘superglue’ for proteins – spontaneously forming irreversible isopeptide bonds with each other when combined. SpyTag/Sp圜atcher and SnoopTag/SnoopCatcher are powerful bioconjugation tools developed by Oxford researchers by exploiting the natural properties of Streptococcus pyogenes and S. By using SnoopLigase in combination with the SpyTag/Sp圜atcher system, researchers can construct biomaterials and enhance enzyme stability, in addition to the diverse uses of the powerful SpyTag/Sp圜atcher system, which include tagging biophysical probes to specific proteins, conjugating antigens to virus-like particles for vaccination, and linking multiple enzymes into pathways to promote metabolic efficiency. The invention maximises the potential of the Tag/Catcher system and renders experimental design and the usage of the system more flexible. This technology addresses the limitation of the widely used Oxford ‘bacterial superglue’ technology – SpyTag/Sp圜atcher system – which is mainly caused by the size of the Catcher – the protein partner of the Tag peptide. To enhance current bioconjugation technology, Oxford researchers have developed SnoopLigase, a catalyst for the formation of a covalent link between two peptides – SnoopTag and DogTag.
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